🔬 Confluent Lysis

Discussion

<p>Note that one means by which phage stocks may be prepared in fact involves phage-class="bap-term">bacterial interaction as it occurs on a plate, a so-called plate lysate, and successful phage wikipedia.org/wiki/Propagation" target="_blank" class="bap-term">propagation in this case generally will result in confluent lysis. Alternatively, dripping of condensation onto a bacterial lawn can result in sufficiently rapid phage diffusion within the droplet, prior to its drying, that again confluent lysis can occur, though generally not across the entire plate.

Stent (1963), p. 182, states: "The multiplication of the phages on this plate produces 10⁵ plaques, so that after incubation the entire surface of this plate will consist of nothing but plaques, or show confluent lysis. However, on such a confluent lysis plate there will generally remain a few bacterial colonies, which can be picked and restreaked through several passages on fresh nutrient agar to free the cells from any contaminating [phages] carried over from the original confluent lysis plate.” (Emphasis in original)

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