by Stephen T. Abedon Ph.D. Steve Abedon, Ph.D., has taught well over 100 individual classes and ~10 individual courses (including introductory biology, microbiology, physiology, biochemistry, etc.) over his 30+ years as a college professor. He has over 150 publications, including multiple monographs, and is ranked in the top 1 or 2 percent among researchers of all disciplines worldwide. His research focuses on bacteriophage biology and phage therapy. (abedon.1@osu.edu)
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phage.org/terms/direct_plating_plaquing_method.html · Abedon’s Books · DOI: 10.5281/zenodo.20173633
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Direct plating generally contrasts with soft-agar overlay or, indeed, simply the pour plate methods as has occasionally also been used. For review of direct plating as a method, see Mazzocco et al. (2009).
The direct-plating plaquing method is the original means by which phage plaques were grown but was subsequently replaced in most but not all labs and systems by the soft agar overlay method. Use of this softer agar, if nothing else, allows for phage diffusion within lower density agar media (i.e., semisolid rather than solid) and also allows for potentially more consistently or easily achieved bacterial lawn homogeneity.
Abedon, S.T. (2026). Phage Terms. https://terms.phage.org · 10.5281/zenodo.20173633
Every term in the Glossary of Bacteriophage Terms. The current page is highlighted purple.