๐Ÿ”ฌ Direct-Plating Plaquing Method

Means of phage growth and biology" class="bap-term">enumeration that involves both phage and bacterial application to the surface of an agar slab as found within a Petri dish.

by Stephen T. Abedon Ph.D. (abedon.1@osu.edu)

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About this entry: Direct-Plating Plaquing Method is one of a growing set of phage biology and phage therapy terms defined here. For more phage terms, see Abedon, S.T. (2025). Phage Therapy Annotated Glossary. Preprints.org. 10.20944/preprints202508.0347.v1

phage.org/terms/direct_plating_plaquing_method.html  ·  Abedon’s Books  ·  DOI: 10.5281/zenodo.20173633

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Direct-Plating Plaquing Method: Means of phage growth and biology" class="bap-term">enumeration that involves both phage and bacterial application to the surface of an agar slab as found within a Petri dish.

Discussion

<p>The direct-plating plaquing method is the original means by which by which phage plaques were grown but was subsequently replaced in most but not all labs and systems by the soft agar overlay method which, if nothing else, allows for phage diffusion within lower density agar media (i.e., semisolid rather than solid) and also allows for potentially more consistently or easily achieved bacterial lawn homogeneity.

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How to cite this page

Abedon, S.T. (2026). Phage Terms. https://terms.phage.org  ·  10.5281/zenodo.20173633

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Direct-Plating Plaquing Method — terms.phage.org — phage.org — Stephen T. Abedon — Version 2026.05.14