🔬 Plaque Morphology

Description of differences especially in the turbidity of phage-induced clearings of bacterial lawns though also the size of those clearings.

by Stephen T. Abedon Ph.D. Steve Abedon, Ph.D., has taught well over 100 individual classes and ~10 individual courses (including introductory biology, microbiology, physiology, biochemistry, etc.) over his 30+ years as a college professor. He has over 150 publications, including multiple monographs, and is ranked in the top 1 or 2 percent among researchers of all disciplines worldwide. His research focuses on bacteriophage biology and phage therapy. (abedon.1@osu.edu)

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About this entry: Plaque Morphology is one of a growing set of phage biology and phage therapy terms defined here. For more phage terms, see Abedon, S.T. (2025). Phage Therapy Annotated Glossary. Preprints.org. 10.20944/preprints202508.0347.v1
To share a particular section, use the 🔗 link beside its heading, which copies a web address that opens the page right there. To cite this page: Abedon, S.T. (2026). Phage Terms. terms.phage.org.  ·  DOI: 10.5281/zenodo.20173633

phage.org/terms/plaque_morphology.html  ·  Abedon’s Books  ·  DOI: 10.5281/zenodo.20173633

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Plaque Morphology: Description of differences especially in the turbidity of phage-induced clearings of bacterial lawns though also the size of those clearings.
Also in the Phage Therapy Annotated Glossary: see the entries “Clear Plaque” (p. 18) and “Turbid Plaque” (p. 56). Read the Glossary · Abedon, S.T. (2025). Preprints.org. doi:10.20944/preprints202508.0347.v1

Discussion

Plaque morphologies can include (i) bull's eyes, (ii) clear plaques, (iii) sectored or star-shaped plaques, (iv) the plaques of T-even phage wild-type versus (v) rapid lysis mutants (which are small with rougher borders versus larger with smoother borders), and (vi) plaques containing central zones of turbidity as often associated with temperate phages. Plaque halos also contribute to plaque morphology as does the mixed-indicator method.

Plaque morphologies can differ with phage isolate, phage mutations, bacterial properties, and environmental conditions. The latter can include such things as temperature, media chemical makeup, the density of bacteria applied to initiate the lawn, and the density of agar present in media, etc.

References

How to cite this page

Abedon, S.T. (2026). Phage Terms. https://terms.phage.org  ·  10.5281/zenodo.20173633

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