๐Ÿ”ฌ Single-Step Growth

Experiment designed to examine only one round of infection by a population of phages.

by Stephen T. Abedon Ph.D. (abedon.1@osu.edu)

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About this entry: Single-Step Growth is one of a growing set of phage biology and phage therapy terms defined here. For more phage terms, see Abedon, S.T. (2025). Phage Therapy Annotated Glossary. Preprints.org. 10.20944/preprints202508.0347.v1

phage.org/terms/single_step_growth.html  ·  Abedon’s Books  ·  DOI: 10.5281/zenodo.20173633

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Single-Step Growth: Experiment designed to examine only one round of infection by a population of phages.

Discussion

<p>Single-step growth experiments are a means of obtaining information about phage burst size and latent period. With artificial lysis of infected bacteria, the eclipse can be determined as well.

Note that burst size also can be determined via end-point determinations, though with less confidence in the resulting data (since there is no assurance that complete phage population lysis in fact has occurred). Similarly, latent periods can be approximated using lysis-profile assays, though if the goal is to approximate single-step growth then effort must be made to assure that phage secondary adsorption does not occur.

External links

How to cite this page

Abedon, S.T. (2026). Phage Terms. https://terms.phage.org  ·  10.5281/zenodo.20173633

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Single-Step Growth — terms.phage.orgphage.org — Stephen T. Abedon — Version 2026.05.14